Biologics manufacturing

Background

Many different types of eukaryotic systems are used to manufacture biologics, where the therapeutic protein is expressed to maximum levels in a production cell, e.g. CHO cells or yeast. Highly active promoters are required to maximise expression and achieve commercially viable milligrams per litre upstream yields.

Why Synthetic Promoters

Not all proteins can be efficiently expressed using the limited selection of promoters available to the industry. Also, some complex biologics are comprised of multiple polypeptides that make up the therapeutic protein. Therefore, it takes many months to empirically create production cells that express the levels of protein required at the correct ratio to make functional protein.

Our Solution

ChromatinBio design strong synthetic promoters that are customised to the production environment. By analysing the genome of the production cell, we can create synthetic promoters optimised to mediate high levels or regulatable expression in the bioreactor. We can also create a collection of promoters that have different levels of activity to allow expression of multiple different polypeptides to the correct stoichiometry.